An automatic queue system has been introduced with fans needing to be online before the start of the sale with a valid registration number.
Around 210,000 people attended the Festival in June, with general tickets for the event selling out in under an hour.
Coldplay, Dua Lipa and SZA played the Pyramid Stage, with Shania Twain performing the Sunday Legends spot.
Registration for 2025 tickets closed at 17:00 on 11 November and fans can buy a maximum of six tickets per person.
Tickets must be fully paid for by the end of 7 April 2025. Any not paid for by then will be put up for resale.
Organisers are keen to encourage people to use environmentally-friendly travel options, with combined ticket and coach packages going on sale earlier this week.
The artists playing Glastonbury 2025 are yet to be announced.
