The time trial is held on an open road course with an elevation gain of 150m (492ft).
It is an amateur event open to anyone with a traditional or modern penny farthing with a wheel diameter in excess of 116cm (46 inches).
The quickest riders are expected to take just over 13 minutes to reach the finish line at Beachy Head.
Between 25 and 30 riders have signed up to compete.
A spokesperson for Visit Eastbourne said: “This event is free to watch and we encourage spectators to cheer on the riders from the pavements along the course.”
