Asked if Lord Alli had received anything from her in exchange for his donations, Rayner said: “I promised nothing and gave him nothing in return.”
The relationship between Labour and Lord Alli has come under scrutiny, after it emerged in August he had been given a temporary Downing Street pass, despite having no official government role.
Asked who signed off the pass for Lord Alli, Rayner said she did not know “but it certainly wasn’t because of any donations that he’d given to me”.
Prime Minister Sir Keir Starmer and his wife Victoria are among those who have received thousands of pounds worth of gifts, including clothing, from Lord Alli.
Following criticism, a Downing Street source said on Friday Sir Keir, Rayner and Chancellor Rachel Reeves would not accept any further donations for clothing.
However, the row risks overshadowing the party’s annual conference in Liverpool, which began on Sunday.